Extended Data Fig. 1: MHC-I genes harbour bivalent H3K4me3 and H3K27me3 modifications.
From: Targeting Menin disrupts the KMT2A/B and polycomb balance to paradoxically activate bivalent genes
a, Genomic snapshots of MHC-I genes showing H3K4me3 and H3K27me3 CUT&Tag in K-562 and ChIP–seq in neuroblastoma KELLY cell lines. The K-562 tracks are also shown in the control cells in Fig. 2h and H3K27me3 control cells in Fig. 6f. b,c, Cell surface MHC-I in K-562 (left) and KELLY (right) cells following treatment with EPZ-011989 and (c) ± 10 ng ml−1 IFN-γ (48 h K-562; 24 h KELLY). d, Genomic snapshots of MHC-I genes showing ChIP–seq for H3K4me3, H3K27me3 and H3K27ac in KELLY cells treated with ethanol (control) or EPZ-011989 ± IFN-γ. e,f, ChIP reChIP–seq of single H3K27me3, single H3K4me3 and reChIP (H3K27me3 and H3K4me3) in K-562 cells. e, Genomic snapshots of bivalent MHC-I genes. f, Heatmaps show bivalent genes −3 kb TSS/+3 kb TES, with genomic regions ordered by H3K27me3 read density in the single H3K27me3 ChIP sample. b,c, Representative plots from three experiments (Supplementary Fig. 3).
