Extended Data Fig. 7: Competition between STAM2A and SOCS-1 for binding to the TYK2–IFNAR1 signalling complex. | Nature Cell Biology

Extended Data Fig. 7: Competition between STAM2A and SOCS-1 for binding to the TYK2–IFNAR1 signalling complex.

From: STAM and Hrs interact sequentially with IFN-α Receptor to control spatiotemporal JAK–STAT endosomal activation

Extended Data Fig. 7

Representative live-cell micropatterning experiment with micropatterned receptor HaloTag–mTagBFP–IFNAR1 as the bait protein (cyan), and TYK2–meGFP FERM–SH2 (FS) and full-length (FL) forms (green), STAM2A–mCherry (yellow) and SOCS-1–iRFP (red) as prey proteins. Scale bars, 10 µm. Interactions of TYK2, STAM2A and SOCS-1 with micropatterned IFNAR1 were quantified according to the contrast within micropatterned cells. Cells expressing TYK2-FS/SOCS-1 (n = 10), TYK2-FL/SOCS-1 (n = 15) or TYK2-FL/SOCS-1/STAM2A (n = 20) were analysed for contrast values and visualized in a box-and-whisker plot (representing the median, minima, maxima and the four quartiles); data were pooled from two independent experiments. Statistical significance was determined using a two-sample Kolmogorov–Smirnov tests. **P < 0.01. Source numerical data are provided.

Source data

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