Fig. 1: Wound memory in HF lineages and its spatial extension.

a, Left: repairing HFs in wound proximity and the HF-derived newly formed epidermis, focus of the study by Gonzales et al.¹⁵. Right: cells in distal HFs and their adaptation programme to wound are the focus of this study. IFESCs: interfollicular stem cells. b, Two-consecutive-skin-injury model: −1w, GL; 0w, homeostasis and first injury; 1w pw1, 1 week post first wound; 2w pw1, 2 weeks post first wound; 8w pw1, 8 weeks post first wound, and second injury; 1w pw2, 1 week post second wound. c, Localizations of Lrig1⁺, Lgr5⁺ and Gata6⁺ cells (left). Epidermal whole-mounts of GL tdTomato⁺ cells (red channel) exiting from HFs (right). HG: hair germ; INFU: infundibulum. d–g, Whole-mounts of Lrig1 GL engaged HFs (red channel). Capital letters define four zones: A, up to 1 mm from wound; B, 1 to 3 mm; C from 3 to 5 mm; D from 5 to 7 mm. Dashed red lines highlight representative engaged HF triplets (d). Number of engaged HFs. n = 6 (0w and 8w pw1), n = 9 (1w pw1 and 1w pw2) (e). Representative pictures of HF engagement in the four zones (A, B, C and D) at 1w pw1 and 1w pw2 (f). Images showing the localization of Lrig1 GL cells in zone D. Asterisks represent Lrig1 GL fibroblasts. White arrow indicates GL cells exiting into IFE (g). h,Top: epidermal whole mounts showing the closure of a second overlapping wound (1w pw2_Over) (left) or of a distal injury (1w pw2_Distal), made in zone B (right). Bottom: quantification of distance from wound centre. n = 4 (1w pw2_Over), n = 5 (1w pw1) and n = 6 (1w pw2_Distal). Yellow arrows indicate wound position (f and g). Dashed circles indicate wound perimeter at 8w pw1 (light blue), 0w (orange); lines underline the migration front of GL cells at 1w pw2 (purple) or 1w pw1 (green) (c and h). P-value from a two-tailed t-test. Data are mean ± s.d. Scale bars: 1 mm (c, d and h); 100 µm (f and g).

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