Fig. 2: Transcriptome of Lrig1 GL cells predicts a new primed subpopulation arising after wound resolution.
From: Tissue memory relies on stem cell priming in distal undamaged areas
a, Memory genes (logFC (1w pw2) > logFC (1w pw1)) and Venn diagram reporting the number and the percentage of inferred memory genes over the total DEGs across the time course in Lgr5 and Lrig1 GL cells. n = 3 mice. b, Enriched GO terms for memory genes in Lrig1 GL cells, as −log10 of p-value. Dashed line indicates significance. n = 3 mice. c, Ex vivo and in vitro experimental settings at 8w pw1: Lrig1 GL skin biopsies from wound-educated Distal region and from Ctrl area, outside from memory zone are compared. For in vitro assays the biopsies were dissociated, and cells were plated. d, Images of ex vivo migration of Lrig1 GL cells (left) and quantification of the migration (exit length) (right). Dashed lines mark the migration front of epidermal cells. Scale bar: 2 mm. Data are mean ± s.e.m. n = 9 skin explants. e,f, Time lapse migration assay in vitro. Track displacement (μm) (e) and representative normalized start position graph (f) of cultured Lrig1 GL cells. Data are mean ± s.d. n = 4 mice. g, RNA-seq of cultured Lrig1 GL cells from wound-educated distal region (Distal 3–7 mm from wound bed (n = 6)) and Ctrl (distance >2 cm from wound bed (n = 8)). The GO analysis relative to upregulated genes in Distal is reported, as −log10 of adjusted p-value (AdjP). Dashed line underlines significance. h, Circular ideogram plot (CIRCOS) of shared DEGs (grey) between 1w pw1, 8w pw1 and 1w pw2 (green) or between 1w pw1 and 1w pw2 only (yellow), in Lgr5 and Lrig1 GL skins. P-value from a two-tailed t-test.
