Fig. 3: Identification of wound-primed cells in the INFU with a pre-activated transcriptional programme.

a, UMAP of scRNA-seq data of Lrig1 GL cells at 0w (red), 1w pw1(green), 8w pw (light blue) and 1w pw2 (purple). b, Unsupervised clustering of single-cell transcriptomic data. c, Summary illustrating epidermal lineages and differentiation in Lrig1 GL single-cell data. Cells are coloured according to their epidermal lineages. Dashed line identifies the homeostatic compartment boundary between upper and lower HF¹¹. d, Expression plot of gene set from Joost et al. study²⁶. e, Pseudotime analysis. Trajectory D is coloured by timepoints and clusters. f, Epidermal whole mounts of Lrig1 GL tdTomato⁺ cells occupancy showing cell localization in the INFU (white arrows) in the distal HFs at 8w pw1 (at 5 mm from wound site). Asterisks mark differentiated IFE cells. g, Plot of the average z-score of the top 100 markers of Transition Cluster 7 showing the intermediate transcriptional state at 8w pw1. h, INFU whole-mount pictures of Krt6 at 0w and 8w pw1 at 5 mm from wound site (left) and quantification at 8w pw1 (right), in wound bed (Wb), distal memory region (Distal 3–7 mm from Wb) or from a naïve region (Ctrl >2 cm from Wb). Ratio to 0w is plotted. Dashed lines indicate 0w average. Data are mean ± s.d. n = 3 mice. i, Spatially resolved scRNA-seq analysis of upper-HF Lrig1 GL cells from wound bed (Wb), distal memory region (Distal 3–7 mm from Wb) and naïve region (Ctrl >2 cm from Wb) was performed at 8w pw1 and analysed in Extended Data Fig. 5. Plot of average z-score expression of the 100 markers of the Transition Cluster identified in Fig. 3g in the cells from each group. n = 42 (Wb), n = 48 (Distal), n = 59 (Ctrl) cells. P-value from a two-tailed t-test. Scale bars: 100 µm (f and h). Data are median with 25th and 75th percentiles if not differently indicated. scRNA-seq data (a–e, g and i) are the integration of two independent experiments, each of them based on four biological replicates.

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