Extended Data Fig. 5: Domain mapping of the interaction of ILF3i1 and WDR24/WDR59.
From: Genome-wide CRISPR screens identify ILF3 as a mediator of mTORC1-dependent amino acid sensing
a, Immunoblotting analysis of cell lines expressing near endogenous levels of GFP-tagged ILF3i1 or ILF3i2. b, HEK293T cells transfected with the indicated domains of ILF3i1 were subjected to immunoprecipitation with FLAG beads. The WCLs and IPs were immunoblotted to detect the indicated proteins. c,d, HEK293T cells transfected with the ILF3i1 CTD and different domains of WDR59 (c) or WDR24 (d) were subjected to immunoprecipitation with HA beads. The WCLs and IPs were immunoblotted to detect the indicated proteins. e, HEK293T cells expressing shILF3 and the indicated domains of ILF3i1 were starved of amino acids for 1 hr. Cell lysates were immunoblotted to detect the indicated proteins and phospho-proteins. f, HEK293T cells transfected with the indicated cDNAs were subjected to immunoprecipitation with anti-HA or anti-FLAG beads. The WCLs and IPs were immunoblotted to detect the indicated proteins. g, HEK293T cells stably expressing Flag-tagged Metap2 or ILF2 were starved of amino acids for 50 min, or starved and re-stimulated with amino acids for 10 min, and then subjected to immunoprecipitation with anti-FLAG beads. The WCLs and IPs were immunoblotted to detect the indicated proteins. Unprocessed blots are available in source data.
