Extended Data Fig. 9: ILF3 plays an evolutionarily conserved role in regulating mTORC1 and autophagy.
From: Genome-wide CRISPR screens identify ILF3 as a mediator of mTORC1-dependent amino acid sensing
a, Summary of the evolutionary conservation of ILF3 and ILF2, as well as GATOR1, GATOR2 and KICSTOR components, in model organisms. b, Quantification of the colocalization between mTOR and TMEM192-EGFP in Fig. 6b using Pearson’s correlation coefficient. Quantification was carried out on 40 cells examined over 3 independent experiments. Data are mean ± s.d. ****P < 0.0001 (unpaired two-sided Student’s t-test). c, HEK293T cells expressing Ctrl or ILF3 shRNAs were starved of amino acids for the indicated times. Cells were subjected to lysis for immunoblotting analysis of the indicated proteins and phospho-proteins. d, HEK293T cells expressing Ctrl or ILF3 shRNAs were starved of amino acids for the indicated times. Cells were subjected to Q-PCR analysis of SQSTM1 mRNA abundance. n = 3 biologically independent experiments. Data are mean ± s.d. n.s., not significant (unpaired two-sided Student’s t-test). Source numerical data and unprocessed blots are available in source data.
