Extended Data Fig. 4: Effect of salinazid treatment on lifespan and number of lipid droplets.
a, Triglyceride abundance among all lipids in middle-aged worms following ash-2 depletion; n = 6 independent biological replicates examined in one experiment. Box-and-whisker plot, with median (central line), 25th and 75th percentile (outer lines) and minimum and maximum within 1.5× the interquartile range (whiskers). Each dot represents one biological replicate. P values: two-tailed Wilcoxon test with Benjamini–Hochberg test for multiple hypothesis correction. b, Salinazid and fat-2 depletion act in the same pathway to extend lifespan; n ≥ 111 worms for each condition. Percentages of median lifespan extension and P values are indicated. P values: log-rank Mantel–Cox test. c, Intestinal lipid droplets, measured by fluorescence, in dhs-3p::dhs-3::GFP worms following Salinazid treatment; n = 29 and 26 worms treated with and without salinazid, respectively. Data are the mean ± s.d. Each dot represents the lipid droplet number in a 26 × 26 µm2 area in the intestine of an individual worm. P values: two-tailed Mann–Whitney test. d, Lpin-1 is necessary for longevity following salinazid treatment; n ≥ 105 worms for each condition. Analysis as in b. b–d, Representative of two independent experiments. Source numerical data of all experiments, replicates and statistics as well as Cox proportional hazard interaction values are provided.
