Fig. 3: Increased lipid droplet number is necessary for MUFA-induced longevity and sufficient to extend lifespan.
a, Schematic of conserved proteins involved in lipid droplet synthesis and degradation. Mammalian protein names are indicated first. Created with BioRender.com. b,c, Number of intestinal lipid droplets, measured by fluorescence, in dhs-3p::dhs-3::GFP worms following lpin-1 depletion and MUFA accumulation. b, Number of lipid droplets in n = 16, 20, 23 and 19 worms treated with control, ash-2, lpin-1, and ash-2 + lpin-1 RNAi, respectively (Extended Data Fig. 2c for the efficiency of the double knockdown). c, Number of lipid droplets in n = 35, 29, 35 and 32 worms treated with control or dietary oleic acid supplementation in the absence or presence of lpin-1 RNAi, respectively. d, lpin-1 is necessary for longevity following ash-2 depletion; n ≥ 96 worms for each condition. e, lpin-1 is necessary for longevity following dietary supplementation with oleic acid; n ≥ 105 worms for each condition. f, Number of intestinal lipid droplets, assessed by fluorescence, in dhs-3p::dhs-3::GFP worms following seip-1 and ash-2 depletion. Zoomed-in images of the intestine. Scale bar, 5 µm. Lipid droplet numbers are provided in Extended Data Fig. 2e. g, seip-1(gk5008) is necessary for longevity following ash-2 depletion; n ≥ 64 worms for each condition. h, seip-1(gk5008) is necessary for longevity following fat-2 depletion; n ≥ 90 worms for each condition. i, Number of intestinal lipid droplets, measured by fluorescence, in dhs-3p::dhs-3::GFP worms following hosl-1 depletion; n = 28 and 25 worms treated with control and hosl-1 RNAi, respectively. b,c,i, Each dot represents the number of lipid droplets in a 26 × 26 µm2 area of the intestine of an individual worm. Data are the mean ± s.d. P values were determined using a two-tailed Mann–Whitney test. j, hosl-1 depletion is sufficient to extend lifespan; n ≥ 94 worms for each condition. d,e,g,h,j, Percentages of median lifespan extension and P values are indicated. P values were determined using a log-rank Mantel–Cox test. b–e,g–j, Data are representative of three (b–e,g,i,j) or two (h) independent experiments. NS, not significant; WT, wild-type worms. Source data are provided.
