Fig. 5: Lipidomic datasets of MUFA-enriched worms reveal changes in membrane lipids and predict decreased lipid oxidation.
a, Untargeted lipidomic analysis on whole worms using LC–MS/MS. b, Principal component (PC) analysis of the lipidome separates MUFA-enriched conditions (ash-2 RNAi) from control conditions. c, Fatty acyl chain abundance of saturated fatty acids (SFAs), MUFAs and PUFAs among all lipids in middle-aged worms following ash-2 depletion. d, Lipidomic analysis of MUFAs and PUFAs in membrane lipids (phospholipids) following ash-2 depletion. e, Lipidomics analysis of ether lipids following ash-2 RNAi depletion. f, Schematic indicating that membrane lipid oxidation is generally increased in the presence of PUFA-containing phospholipids and ether lipids, particularly those present in PUFAs. Accumulation of lipid oxidation can lead to membrane damage and loss of membrane integrity. g, Lipidomic analysis of the peroxidation index (probability of lipid oxidation; calculation in Methods) following ash-2 depletion. a and f are created with BioRender.com. b–e,g, Each dot represents a biological replicate; n = 6 independent biological replicates examined in one experiment. c–e,g, Box-and-whisker plots with the median (central line), 25th and 75th percentiles (outer lines), and minimum and maximum within 1.5× the interquartile range (whiskers) indicated. P values were determined using a two-tailed Wilcoxon test with Benjamini–Hochberg test for multiple hypothesis correction. Source data are provided.
