Fig. 6: MUFAs decrease age-dependent lipid oxidation and preserve cell and membrane integrity.
a, Relative MDA levels, used as a measure of the level of lipid oxidation, during aging and following ash-2 depletion. Data for n = 4, 13 and 11 samples from control young, control middle-aged (control RNAi) and middle-aged worms treated with ash-2 RNAi, respectively, were normalized to the middle-aged control of the corresponding experiment. b, Lipid oxidation assessed via western blotting for 4-HNE levels during aging and following salinazid treatment. The arrows indicate bands that change following salinazid treatment. c, Levels of lipid oxidation, quantified via MDA levels, following supplementation with oleic acid or elaidic acid; n = 10, 9 and 6 samples from middle-aged worms with control, oleic acid and elaidic acid supplementation, respectively. d,e, Cell and membrane integrity, assessed according to the intensity of propidium iodide (PI) staining, during aging and following ash-2 depletion. d, Images of one worm per condition stained with PI. Dashed lines outline the worms. Scale bar, 100 µm. e, Intensity of PI staining for n = 32, 40, 40 and 55 young and old worms treated with control RNAi, old worms treated with ash-2 RNAi and dead worms, respectively. Each dot represents the mean PI signal in one worm. f, Levels of lipid oxidation, quantified via MDA levels, following oleic acid supplementation and lpin-1 depletion; n = 10, 9, 8 and 8 samples from middle-aged worms with control or oleic acid supplementation in the absence or presence of lpin-1 RNAi, respectively. g, Levels of lipid oxidation, quantified via MDA levels, in seip-1 mutant worms; n = 8 samples from middle-aged worms for each condition. h, Levels of lipid oxidation, quantified via MDA levels, following salinazid treatment; n = 9 samples from middle-aged worms for each condition. a,c,f–h, Each dot represents a biological replicate. Each shape represents an independent experiment. a,c,f–h, Data are the mean ± s.d. of four (a), two (c,f,g) or three (h) independent experiments. P values were determined using a two-tailed Mann–Whitney test. i, Salinazid and ash-2 depletion act in the same pathway to extend longevity. Percentages of the median lifespan extension and P values, determined using a log-rank Mantel–Cox test, are indicated; n ≥ 110 worms for each condition. b,e,i, Data are representative of two (b) or three (e,i) independent experiments. Source data are provided.
