Extended Data Fig. 4: Accumulation of p16INK4a expressing cells in other tissues with ageing.
From: Bacterial induction of B cell senescence promotes age-related changes in the gut microbiota
a, The bioluminescence imaging with a lowered threshold for the data in Fig. 1a shows an increase in signal with ageing in mesenteric lymph nodes, spleen, liver and colon. The colour bars indicate the radiance with minimum and maximum threshold values. b and c, The bioluminescence intensity emitted from the lungs, mesenteric lymph nodes, spleen, liver, and colon of 6 M or 20 M SPF or GF mice (b) or the expression level of p16INK4a in those tissues (c). The sample size (n) represents the number of biologically independent animals (b, c, n = 5). Data are presented as mean values ± s.e.m. Statistical significance was determined with two-way ANOVA followed by Ĺ Ădák’s multiple comparisons test (b, c). All experiments were repeated at least twice, independently, with similar results. NS, not significant. Numerical data is available in source data.
