Extended Data Fig. 5: Gating strategy of B cells in flow cytometry.
From: Bacterial induction of B cell senescence promotes age-related changes in the gut microbiota
Total lymphocytes isolated from Peyer’s patches or lamina propria of small intestine were gated on a forward scatter (FSC)/side scatter (SSC) plot and then gated on the Zombie-NIR– population to remove dead cells. These cells were further gated for the B cell subsets of interest, namely IgA plasma cells (B220– IgA+), germinal center (GC) B cells (B220+ PNA+ FAS+), or non-GC B cells (B220+ PNA– FAS–). Data were analyzed using FlowJo software, and percentages in the figure represent the frequency in the parent population.
