Extended Data Fig. 9: Multiomic sequencing comparison of week 2 in vitro SC-islets with 6 months in vitro SC-islets and 6 months in vivo SC-islets.
a, Integrative UMAP clustering showing cells from 2 weeks in vitro SC-islets, 6 months in vitro SC-islets, and 6 months in vivo SC-islets using gene expression and chromatin information (24491 cells from 5 independent biological samples; 1 representative week 2 SC-islets, month 6 SC-islets, 3 month 6 in vivo SC-islets). b, Heatmap showing gene expression and promoter accessibility of gene markers for SC-β, SC-α, SC-δ, and SC-EC cells. c, Gene expression and motifs accessibility of active transcription factors associated with SC-β, SC-α, and SC-δ identity. d, Gene list showing markers for β cell identity 6 months in vitro, in vivo or both. Initial number of genes represent upregulated genes in the 6 month conditions (vitro and vivo) when compared to 2 week SC-islets. List of genes was cross-referenced with β cell upregulated genes from primary human islets. e, Plot highlighting greater increase of motifs accessibility from 6 months in vivo SC-β cells when compared in 6 months in vitro.
