Extended Data Fig. 5: The ER makes more contacts with keratin filaments compared to microtubules proximal to the desmosome.
From: Architecture and dynamics of a desmosome–endoplasmic reticulum complex
(a–b) FIB-SEM segmentations showing keratin filaments (teal), desmosomes (yellow), microtubules (orange), and ER (magenta) (A). Panel (B) displays ER-keratin (teal) and ER-microtubule (orange) contacts at a distance threshold of 16 nm. Scale bar = 1μm. (c–f) Close-up of keratin filaments, microtubules, and ER adjacent to a desmosome (C-E) revealing larger and more numerous ER-keratin contacts (blue arrowheads) compared to ER-microtubule contacts (orange arrowheads) (F). Images in A-F are representative of n = 1 cell-cell contact. Scale bar = 500 nm. (g) Violin plots of ER-keratin (blue) and ER-microtubule (orange) contacts at x nm away from the closest desmosome. Individual contacts are represented by black vertical lines. Setting various contact site distance thresholds between 0 nm to 16 nm (y-axis) demonstrates that ER-keratin contacts preferentially occur proximal to desmosomes. Source numerical data are available in source data.
