Extended Data Fig. 8: ER tubules associate with desmosomes during fusion events.
From: Architecture and dynamics of a desmosome–endoplasmic reticulum complex
(a–f) Snapshots of a live-cell time-course of desmoplakin (top row, orange in bottom row) and VAPB (middle row, magenta in bottom row) in A431 cells during the fusion of DP puncta at the cell-cell contact (Images are representative of n = 4 independent experiments). Three DP puncta (A) fuse to form two puncta (B). Eventually, these puncta fuse to form one DP puncta (F). Gamma correction was applied to grayscale images. Gamma = 0.7 (DP/ VAPB merge), Gamma (inverted) = 1.5 (VAPB). Scale bar = 1μm. (g–l) ilastik-segmentations of DP (top row) and VAPB (middle row) channels in A-F. Bottom row indicates overlapping pixels (white) between DP and VAPB channels. Overlap indicates that tips of ER tubules colocalize with DP puncta during fusion. Scale bar = 1μm.
