Extended Data Fig. 9: ER tubules remain at the cell-cell border even when desmoplakin translocation to the border is inhibited.
From: Architecture and dynamics of a desmosome–endoplasmic reticulum complex
(a–e) Representative time-lapse of A431 cells expressing DP-EGFP (orange) and mApple-VAPB (magenta) pre-treated with DMSO for 30 minutes, followed by a switch to high calcium media. ER tubules extend toward the cell periphery, sometimes forming paired structures across adjacent cells (red arrows, B). Desmosomes form at the exact position of ER paired structures (blue arrows in E). Images are representative of n = 2 independent experiments. Scale bar = 2μm. (f–j) Representative time-lapse of A431 cells expressing DP-EGFP (orange) and mApple-VAPB (magenta) pre-treated with 1μM thapsigargin (TG) for 30 minutes, followed by a switch to high calcium media. ER tubules extend toward the cell periphery, sometimes forming paired structures across adjacent cells (red arrows, F, G). ER tubules eventually remain at the periphery in adjacent cells, but no desmosomes form (J). Timestamps indicate time after DMSO/ thapsigargin wash-out and switch to high calcium media. Images are representative of n = 3 independent experiments. Scale bar = 2μm.
