Extended Data Fig. 10: Keratin filaments and aggregates are stably tethered to ER membranes.
From: Architecture and dynamics of a desmosome–endoplasmic reticulum complex
(a) Snapshot of an A431 cell expressing mNeonGreen-KRT14WT (blue) and mApple-VAPB (magenta) showing ER tubules along keratin filaments (Images are representative of n = 3 independent experiments). Solid yellow line indicates position of kymograph in B-D. Scale bar = 1μm. (b-d) Kymograph of yellow line in A revealing stable ER-keratin interaction over a 2-minute time course. Scale bar = 500 nm. (e) Snapshot of an A431 cell expressing mNeonGreen-KRT14R125C (blue) and mApple-VAPB (magenta) showing ER sheets surrounding KRT14R125C aggregates (Images are representative of n = 3 independent experiments). Solid yellow line indicates position of kymograph in F-H. Scale bar = 1μm. (f–h) Kymograph of yellow line in E revealing stable ER-keratin interactions over a 2-minute time course. Scale bar = 500 nm. (i) Graph depicting the fraction of KRT14R125C aggregates colocalizing with ER at each timepoint during a 25 timepoint/2-minute time course. Data are represented as mean ± s.d.; n indicates total number of KRT14R125C aggregates analyzed from three independent experiments. Source numerical data are available in source data.
