Extended Data Fig. 1: ER tubules are proximal to desmosomal junctions in various epithelial cell culture models.
From: Architecture and dynamics of a desmosome–endoplasmic reticulum complex
(a–c) Representative light microscopy images of a pair of A431 cells (A), HaCaT immortalized keratinocytes (B), and primary normal human epidermal keratinocytes (C) expressing mCherry-VAPB (magenta, ER marker) showing ER tubule and desmosomal junction (orange) proximity (red or white arrowheads). Desmosomes are labelled with DP-EGFP in A and B, or with an Alexa Fluor 488 conjugated anti-DSG3 mAb (AK23) in C. Images are representative of n = 3 independent experiments (A, B) and 2 independent experiments (C). Scale bar = 2μm.
