Extended Data Fig. 4: Alteration of presynaptic markers.
From: Autophagy enables microglia to engage amyloid plaques and prevents microglial senescence
Levels of several presynaptic markers, including SV2A, SV2B, VGLUT1, VGLUT2, Synaptophysin, and Synaptogyrin, were determined in 8-months-old mice brains from 5xFAD and WT (a, 5 male and 3 female mice per group), Atg7cKO; 5xFAD (7 male mice) and Atg7WT; 5xFAD (b, 6 male mice), and Atg7cKO (4 male and 4 female mice) and Atg7WT (c, 4 male and 3 female mice). (d) Immunostaining of Lamp1 and VGLUT2 along with APP. Representative image from at least 3 different experiments. Scale bar, 50 µm. p = 0.02 for SV2B (a); p = 0.03 for VGLUT1 (a); p = 0.003 for VGLUT2 (a); p = 0.0042 for VGLUT2 (b). p-values were calculated by unpaired two-tailed Student’s t-test. All values are reported as mean ± SEM. Source numerical data and unprocessed blots are available in source data.
