Extended Data Fig. 3: Osteogenic Notch signaling and dominance of endothelial Vegfr2 over Dll4.
From: Spatial heterogeneity of bone marrow endothelial cells unveils a distinct subtype in the epiphysis
(a) Protocol for 4OHT injection in neonates. P, postnatal. (b–h) Immunohistochemistry of femur sections and whole-mount Alizarin red staining at P11 and quantification (Cont: = 3, Rbpj iΔOsx: n = 4, biologically independent experiments). (i) Protocol for 4OHT injection in neonates. (j–m) Immunohistochemistry of femur sections at P11. Combined deletion of Vegfr2 and Dll4 in ECs shows that the phenotypes resulting from Vegfr2 knockout dominate those resulting from Dll4 knockout (open arrowheads). Scale bars: 1 mm (b, c, f, g, j, k); 200 µm (d, e, l, m). Data presented are the mean ± SD. The comparisons between the averages of two groups were evaluated using the two-tailed Student’s t-test.
