Fig. 6: Type S vessels harbour HSCs.
From: Spatial heterogeneity of bone marrow endothelial cells unveils a distinct subtype in the epiphysis
a, Protocol for 4OHT injection during the second week after birth. b, Immunohistochemistry of femur sections at P18 and quantification (vessel area: n = 3, c-Kit+ cells per FOV: n = 4, biologically independent experiments). Vegfr2iΔEC mice lack c-Kit+ cells especially in the avascular area (asterisks). c, Protocol for tamoxifen injection in adult mice. d, Flow cytometric analysis of BM cells isolated from the epiphysis of 12-week-old mice and quantification (n = 3, biologically independent experiments). Scale bar, 200 µm. **P < 0.01; *P < 0.05. Data presented are the mean ± s.d. The comparisons between the averages of two groups were evaluated using the two-tailed Student’s t-test.
