Fig. 7: Epiphysial HSCs reconstitute long-term multilineage haematopoiesis.
From: Spatial heterogeneity of bone marrow endothelial cells unveils a distinct subtype in the epiphysis
a,b, Flow cytometric analysis of BM cells isolated from the epiphysis and metaphysis of 12-week-old mice (a) and quantification (b) (n = 3, biologically independent experiments). c,d, Quantification of donor-derived cells (c) and lineage differentiation in peripheral blood samples (d) after the first BMT (n = 6, biologically independent experiments). e, Chimaerism of donor-derived cells in the BM (n = 6, biologically independent experiments) at 4 months after the first BMT. f,g, Quantification of donor-derived cells (f) and lineage differentiation in peripheral blood samples (g) after the second BMT (n = 6, biologically independent experiments). h,i, Flow cytometric analysis of BM cells isolated from the epiphysis and metaphysis of 12-week-old mice (h) and quantification (i) (n = 4, biologically independent experiments). The cell-cycle state was determined by DAPI and Ki67 staining. ***P < 0.001; **P < 0.01; *P < 0.05. Data presented are the mean ± s.d. The comparisons between the averages of two groups were evaluated using the two-tailed Student’s t-test.
