Extended Data Fig. 1: Quality control and characterization of endothelial clusters in scRNA-seq.
From: Spatial heterogeneity of bone marrow endothelial cells unveils a distinct subtype in the epiphysis
(a) Statistics of cells analyzed by scRNA-seq. (b) Immunohistochemistry of a femur section mechanically dissected into three fragments: diaphysis (Dia), metaphysis (Meta), and epiphysis (Epi). (c) Violin plots of internal control genes for the comparison of all fragments. (d) A scatter plot of normalized expression and ridge plots of raw UMI (Unique Molecular Identifier) counts for Col1a1 and Col1a2. Both plots confirm that definite populations of type S ECs express these genes abundantly. (e) IPA showing the top four networks represented by genes upregulated in type S ECs. Scale bar: 1 mm. In the box plots of c, the lower and upper bounds of box represent the 25th and 75th percentile, respectively. The box represents the middle 50% of the data, namely interquartile range (IQR). The horizontal line within the box represents median. The lower and upper bounds of whisker represent the the minimum and maximum values within 1.5 times the interquartile range below the 25th percentile and above the 75th percentile, respectively. The black dots represent outliers. Data presented are the mean ± SD.
