Extended Data Fig. 10: LPS-bound EVs colocalize with CD14. | Nature Cell Biology

Extended Data Fig. 10: LPS-bound EVs colocalize with CD14.

From: Host extracellular vesicles confer cytosolic access to systemic LPS licensing non-canonical inflammasome sensing and pyroptosis

Extended Data Fig. 10

a,b, Casp11−/− iBMDMs were stimulated with FITC-LPS-EVs unlabeled (a) or labeled with CellBrite Steady 550 dye (b). After 4 h, cells were stained with anti-CD14, anti-EEA1, and anti-FITC antibodies and subjected to confocal imaging. In (b), light blue arrows indicate colocalization of FITC-LPS-EVs (CellBrite-labeled) with CD14 (top two rows) and their internalization (bottom two rows). Images are representative of two experiments. Scale bar, 5 µm (a,b).

Back to article page