Extended Data Fig. 2: Dynamic changes of lipidome in mouse preimplantation embryo development using the Bligh & Dyer method.
From: Low-input lipidomics reveals lipid metabolism remodelling during early mammalian embryo development
(a) A PCA plot of the lipidomics profiling data showing clustering of seven biological replicates of oocytes and zygote stage embryos each, four biological replicates of two-cell stage embryos each and five biological replicates of four-cell, eight-cell and blastocyst stage embryos each. (b) Levels of all lipid classes quantified by LC–MS in six developmental stages. Data are from the same biological replicates as in (a) and are presented as the mean +/-s.e.m. (c) A pie chart showing the proportion of each lipid class in every developmental stage. (d) Heatmaps showing the relative abundance of lipids in oocytes, zygotes, two-cell, four-cell, eight-cell and blastocyst embryos. Scaled value bar indicates the relative concentration. Data are from the same biological replicates as in (a).
