Extended Data Fig. 5: The degree of lipid unsaturation is increased during mammalian preimplantation embryo development.
From: Low-input lipidomics reveals lipid metabolism remodelling during early mammalian embryo development
(a) Nightingale rose chart showing the distribution of polyunsaturated, mono+diunsaturated and saturated lipids in different lipid classes. Numbers of species measured in each class are shown. (b-d) Bar graphs showing the polyunsaturated lipids levels of LPCs (b), PC-Os (c) and PSs (d) in the mouse two-cell and blastocyst stage embryos. (e-g) Bar graphs showing the mono+diunsaturated lipid levels of GluCers (e), SLs (f) and SMs (g) in the mouse two-cell and blastocyst stage embryos. (h-l) Box plots showing the polyunsaturated lipid levels of PGs (h), BMPs (i), CLs (j), PC-Os (k) and TAGs (l) in the human eight-cell and blastocyst stage embryos. (m-n) FPKM values of Scd gene (m) and Fads gene (n) transcripts at different developmental stages. Each dot represents one biological replicate. Data are from two biological replicates of oocytes, four biological replicates of two-cell and four-cell stage embryos, three biological replicates of eight-cell stage embryos, two biological replicates of morula stage embryos, four biological replicates of ICM and TE of E3.5 blastocyst stage embryos, and two biological replicates of Epi of E6.5 of post-implantation embryos (ref. 4) and are presented as the mean +/-s.e.m. (o) Bar graphs illustrating the levels of palmitic acid (C16:0), palmitoleic acid(C16:1) and the ratio of C16:1/C16:0 in the mouse two-cell and the blastocyst stage embryos. (p) Box plot showing the ratio of palmitoleic acid (C16:1)/palmitic acid (C16:0) in the human eight-cell and blastocyst stage embryos. (b-g, o) Each dot represents one biological replicate, and data are from four biological replicates of two-cell stage embryos each and three biological replicates of blastocyst stage embryos each and are presented as the mean +/-s.e.m. Statistical significance was determined by two-tailed unpaired t test. (h-l, p) Each dot represents one biological replicate, data are from four biological replicates of eight-cell and blastocyst stage embryos each and are presented as the mean +/-s.e.m. Statistical significance was determined by two-tailed unpaired t test. Center line, median; box, 25th and 75th percentiles, whiskers, 1.5xIQR.
