Extended Data Fig. 6: Inhibition of SCD1 during mouse embryo development.

(a) A schematic of the experimental approach. Zygotes were isolated at 24 h after injection of hCG and cultured in KSOM medium with 100 nM CAY10566 or 100 nM CAY10566 plus 100 nM oleic acid, or Scd1 siRNA injection was performed at the zygote stage, and the embryos were collected at the indicated times for further analysis. (b) TUNEL assay was performed at 3.5 d.p.c embryos cultured in medium with DMSO, 100 nM CAY10566 or 100 nM CAY10566 plus 100 nM oleic acid. Representative images of embryos under the above three conditions are shown. (c) Scd1 mRNA expression at 3.5 d.p.c after Scd1 siRNA injection at the zygote stage. Data are from three independent experiments and are presented as the mean +/-s.e.m. Statistical significance was determined by two-tailed unpaired t test. (d) FPKM values of Elovl gene transcripts at different developmental stages. Each dot represents one biological replicate. Data are from two biological replicates of oocytes, four biological replicates of two-cell and four-cell stage embryos, three biological replicates of eight-cell stage embryos, two biological replicates of morula stage embryos, four biological replicates of ICM and TE of E3.5 blastocyst stage embryos, and two biological replicates of Epi of E6.5 of post-implantation embryos (ref. ⁴) and are presented as the mean +/-s.e.m. (e) Representative images of mouse embryos at 2.5, 3.5 or 4.5 d.p.c that were treated with siRNA of scramble (NC) or Elovl5 from the zygote stage. The images shown are representative of three independent experiments. (f) Elovl5 mRNA expression at 4.5 d.p.c after Elovl5 siRNA injection at the zygote stage. Data are from three independent experiments and are presented as the mean +/-s.e.m. Statistical significance was determined by two-tailed unpaired t test.

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