Fig. 2: Pho81 positively regulates pexophagy during P-S.
From: A metabolite sensor subunit of the Atg1/ULK complex regulates selective autophagy
a,b, Whole cell TMT proteomics analysis of indicated strains compared with WT after P-S (24 h) (n = 3 biologically independent experiments): significant proteome changes in ∆atg1 and ∆atg11 cells compared with WT, where volcano plots show significant fold changes (FC) above horizontal dotted line and top 20 proteins with the highest adjusted P value are labelled (a); significant proteome changes in ∆pho81, ∆atg11 and ∆atg11∆pho81 cells compared with WT (b). c, 2GFP–Atg8, Om45–GFP or Pex11–GFP turnover in WT and ∆pho81 cells upon P-S (24 h). Data are mean ± s.d. (n = 6 biologically independent experiments). d,e, Pex11–GFP turnover in indicated strains after P-S (24 h). Data are mean ± s.d. (d, n = 11; e, n = 3 biologically independent experiments). f, Fluorescence imaging of indicated strains expressing PEX11–GFP after P-S (0 and 24 h). Images represent maximum intensity Z-stack projections. Right: quantification of peroxisomes per cell. Box-and-whiskers plots: the boxes extend from the 25th to the 75th percentile spanning the IQR, whiskers show minimal and maximal values, black line in the middle of the boxes represents the median (n = 100 cells examined over four independent experiments). g, Fluorescence imaging of indicated strains expressing genomic PHO81–GFP and pRS315–BFPeSKL to visualize peroxisomes during growth and after P-S (4 h). Arrowheads indicate Pho81–GFP positive (white) or negative (purple) peroxisomes. Data are representative of three biologically independent experiments. Scale bars, 5 µm (f and g). Statistical significance was assessed using two-sided statistical testing with adjustment for multiple testing within a comparison performed using limma49 (a and b), two-tailed t-test **P = 0.0005 (c), one-way ANOVA followed by Tukey’s multiple comparisons test, P values relative to WT: **P = 0.0033; ***P < 0.001 (d and e), and two-way ANOVA followed by Å Ãdák’s multiple comparisons test, P values relative to WT: ***P < 0.0001 (24 h); P values 24 versus 0 h timepoints: #, P = 0.0319; ##, P = 0.0014; ###, P < 0.0001 (f). Source numerical data and unprocessed blots are available in Source data.
