Fig. 4: Atg13 phosphorylation by TORC1 determines dependence of pexophagy on Pho81 during P-S.
From: A metabolite sensor subunit of the Atg1/ULK complex regulates selective autophagy
a, Principal component analysis of phosphoproteomics data for WT and indicated cells upon P-S (red) and N-S (blue) after 8 h. b, Significantly changed phosphosites during P-S and N-S (grey), WT and ∆pho81 after P-S (red), or WT and ∆atg13 after N-S (blue) after 8 h. c, Phosphorylation of representative TORC1 kinase and Atg1 kinase sites in indicated cells upon P-S and N-S (8 h). Data in a–c are derived from four biologically independent experiments. d, Phosphorylation of endogenous Atg13 in WT and ∆pho81 cells during P-S ± rapamycin or N-S at indicated timepoints. Samples were analysed by whole cell extraction and western blot using an α-Atg13 antibody (n = 3 biologically independent experiments). e, Pex11–GFP turnover in indicated strains after P-S (24 h) ± rapamycin. Data are mean ± s.d. (n = 4 biologically independent experiments). f, Pex11–GFP turnover in indicated strains after P-S (24 h). Data are mean ± s.d. (n = 4 biologically independent experiments). Statistical significance was assessed using two-way ANOVA followed by Tukey’s multiple comparisons test. P values relative to WT-rapa or as indicated *P = 0.0494, **P = 0.0014, ∆pho81 − versus + rapa ***P = 0.0006, ∆atg11 + rapa versus WT − rapa ***P = 0.0004 (e) and one-way ANOVA followed by Tukey’s multiple comparisons test, P values relative to WT, ***P ≤ 0.0002 (f), non-significant (n.s.). Source numerical data and unprocessed blots are available in Source data.
