Extended Data Fig. 1: Additional analysis of Pho81 in autophagy during P-S.
From: A metabolite sensor subunit of the Atg1/ULK complex regulates selective autophagy
a, Fluorescence imaging of strains co-expressing plasmid encoded PHO81-mCherry under ADH1 promoter and genomic 2GFP-ATG8 upon phosphate starvation (4 h). Scale bar = 5 µm. Quantification of Pho81-mCherry puncta and co-localization with 2GFP-Atg8. Data are means ± SD (n = 100 cells examined over 5 independent experiments). b, Y2H of cells harboring pGAD (ev) or pGAD-PHO81 and pGBDU-ATG11 in WT (pJ69-4a) or ∆atg36 cells grown on SD + HIS or SD-HIS media. c, Fluorescence imaging of strains expressing plasmid-encoded PHO81-mCherry variants under ADH1 promoter during growth. Scale bar = 5 µm. Quantification of Pho81-mCherry puncta per cell. Data are means ± SD (n = 125 cells examined over 5 independent experiments). d, Pex11-GFP turnover in cells expressing indicated plasmid-encoded PHO81 variants in Δpho4 or Δpho4Δpho81 cells after phosphate starvation (24 h). Data are normalized to the empty vector control in Δpho4 cells and are means ± SD (n = 6 biologically independent experiments). Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparison test. P-values are relative to WT (c), ev control in Δpho4, or as indicated: *, p < 0.05; **, p < 0.01; ***, p < 0.001. Source numerical data and unprocessed blots are available in source data.
