Extended Data Fig. 3: Condensate motility and phospholipid specificity experiments.
From: Biomolecular condensation orchestrates clathrin-mediated endocytosis in plants
a, Representative time-lapse and tracking of AtEH1 and AtEH2 condensates in Latrunculin B treated (4 μM, 30 minutes) N. benthamiana epidermal cells. b, Schematic representation of the phospholipid localisation domains in Tobacco pollen tubes which maintain a distinctive lipid signature. Images show transient co-expression of pLAT52:AtEH1-YFP with lipid biosensors (pLAT52-biosensor-mCherry) in N. tabacum pollen tubes. The chart shows the quantification of the plasma membrane signal from AtEH1 and lipid biosensors from the tip of the pollen tube. Data represent mean ± SD, quantified from n = 10 time points pooled from two individual pollen tubes (5 timepoints per pollen tube) for each lipid biosensor. Scale bars = 5μm. For a-b the experiments were performed two times with similar results.
