Extended Data Fig. 1: TPC disorder prediction, and AtEH1 in vitro FRAP assay.
From: Biomolecular condensation orchestrates clathrin-mediated endocytosis in plants
a, Plot of the proportion of disordered residues for TPC subunits. b, Plot of TPC subunits showing prediction of disordered (MobiDB, blue), and prion-like (PLAAC, red) residues. Regions with values >0.5 are considered disordered, or prion-like respectively. Prion-like residues were only identified in TML and TWD40-2 subunits. (c,d) In vitro FRAP assay of purified GFP-AtEH1FL protein. Data is mean ± SD, n indicates the number of puncta from an individual experiment that were used in the quantification shown in panel d. Scale bar = 2 μm. The experiment in c-d was performed two times with similar results.
