Fig. 2: WIPI4 loss of function induces ferroptosis in zebrafish.
From: Loss of WIPI4 in neurodegeneration causes autophagy-independent ferroptosis
a, Zebrafish juveniles with wdr45 knockdown (wdr45 KD) by CRISPR injection had reduced lifespans compared with their uninjected siblings from the age of 4 weeks. Survival rate of WIPI4 CRISPR mutants and their uninjected siblings over a period of 7 weeks (n = 60 fish per group); log-rank Mantel–Cox test. b, Treatment of rho:EGFP transgenic fish with 10 μM Fer-1 from 5 to 10 d.p.f. rescued the loss of photoreceptors following wdr45 KD by CRISPR injection, whereas Fer-1 did not cause any change in the fluorescence rod area of uninjected fish. Representative images of sections across the eye of 10 d.p.f. rho:EGFP fish injected with wdr45-targeting CRISPRs and their uninjected siblings treated with DMSO or 10 μM Fer-1, respectively. Scale bar, 50 μm. c, Photoreceptor areas from the images in b; n ≥ 23 eyes per group. d, Increase in lipid peroxidation, represented by higher concentrations of MDA, in wild-type fish subjected to wdr45 KD by CRISPR injection at 5 d.p.f. compared with their uninjected siblings; n = 5 biologically independent experiments, 30 fish each. Data are the mean ± s.d. *P < 0.05 and **P < 0.01; two-tailed unpaired Student’s t-test. Source numerical data are provided.
