Extended Data Fig. 5: Control for mechanical manipulation of the blastocoel.
From: Competence for neural crest induction is controlled by hydrostatic pressure through Yap
a, Schematic showing the puncture procedure of inflation and deflation. Embryos were punctured with a needle similar to the one used for the inflation/deflation experiments, but no liquid was injected or removed from the blastocoel cavity. b, Embryos analyzed via in situ hybridization at stage 15 using snai2 marker. c, Spread of data points indicating the change in neural crest expression in control embryo (n = 10 embryos) and punctured embryo (n = 15 embryos). d, Control embryo compared to embryo treated with ouabain as described in Methods, analyzed at stage 14 using snai2 marker. e, Spread of data points comparing change in neural crest expression between control embryo (n = 9 embryos) and treated embryo (n = 11 embryos). Scale bar 450 µm (b and d). Data represent mean, error bars are s.d. Statistical analysis was performed using a two-sided unpaired student t-test (NS, P = 0.2667; c and ****P ≤ 0.0001; e, CI = 95%).
