Extended Data Fig. 7: Deflation expands neural crest at the expense of epidermis.
From: Competence for neural crest induction is controlled by hydrostatic pressure through Yap
a, Schematic of deflation assay to reduce blastocoel volume. b, Double in situ hybridization of neural plate marker (Sox2, cyan) and neural crest marker (snai2, purple). c, Spread of data points comparing the relative area of the neural plate in control (n = 8 embryos) and deflation (n = 11 embryos). d, In situ hybridization of epidermis marker (keratin, purple). e, Spread of data points of relative area of epidermis in control (n = 10 embryos), and deflation (n = 20 embryos). Embryos in (b) and (d) were analyzed at stage 14. Scale bar 450 µm (b). Data (c, e) represent mean, error bars are s.d. Statistical analysis was performed using a two-sided unpaired student t-test (NS, P = 0.0626; c and **P = 0.0017; c and e, CI = 95%).
