Extended Data Fig. 1: Analysis of neural crest competence.
From: Competence for neural crest induction is controlled by hydrostatic pressure through Yap
a, Schematic of neural crest ectopic induction via DLMZ assay, where stage 10 donor embryo injected with FLDX as a lineage tracer (cyan) in two blastomeres at the 2-cell stage. b, In situ hybridization of snai2 at stage 16, showing embryos with dorso-lateral and ventral ectopic induction. When the grafted tissue was located next to the host neural crest, we observed expansion in the markers, whereas when the graft was ventral, we observed proper ectopic induction; only the latter was considered in the analysis. c, Percentage of embryos exhibiting either ventral or dorso-lateral ectopic induction. d, In situ hybridization of Wnt8 positive animal and ventral sides after grafting the DLMZ into the blastocoel cavity. f, Percentage of embryos exhibiting either positive or negative ectopic Wnt8 expression. Histograms represent mean, error bars are s.d. Panels represent three independent experiments. Scale and 400 µm (b) and 350 µm (d and e). Three independent experiments (c, f).
