Extended Data Fig. 3: Spatiotemporal mapping reveals dynamics of nucleosome and nuclear landmark proteins.

a, Nuclei in G1 cells with minimal drift selected for spatiotemporal mapping. Scale bar: 1.0 μm. b, Localization density calculated using 150 nm detection radius from bins separated by 10 nm. c, 150 nm detection radius and bin distance of 10 nm provide optimal coverage and resolution for the density on the bound map of RNAPII. Scale bar: 0.5 μm (n = 15 cells; Error bar: centroid of nucleolus ± s.d.). d, 3D simulation of the yeast interphase genome based on 3 C data³⁷. e, Top mRNA genes simulation maps based on gene expression data in minimal medium³⁸. Left panel is reuse of Fig. 1d top panel (n: number of genes). f, Freely diffusing NLSx2-Halo map. Scale bar: 0.5 μm (Error bar: centroid of nucleolus ± s.d.; n: number of nuclei). g, Bound Cse4 (left) and Sir4 (right) maps (Error bar: centroid of nucleolus ± s.d.; n: number of nuclei). Sir4 bound map showed dense regions proximal and distal to the centromere, mirroring the telomere clusters of short and long chromosomes^35,37,95. h, Bound H2B diffusion coefficient map. A 300 nm detection radius was used. Scale bar: 0.5 μm (Error bar: centroid of nucleolus ± s.d.; n: number of nuclei). Telomere and centromere movements are confined due to attachment to the nuclear envelope and spindle pole body, respectively⁹⁶. Likewise, the local diffusion coefficients of chromatin-bound H2B reveal lower histone dynamics near centromeres and telomeres (Extended Data Fig. 1h, i).