Extended Data Fig. 10: CTD is necessary but not sufficient for confining protein diffusion. | Nature Cell Biology

Extended Data Fig. 10: CTD is necessary but not sufficient for confining protein diffusion.

From: Disordered C-terminal domain drives spatiotemporal confinement of RNAPII to enhance search for chromatin targets

Extended Data Fig. 10

a, HaloTag (NLSx2-Halo) and Halo-CTD fusions diffusion coefficient histogram by CTD length (n: number of trajectories; mean value ± s.d.). The first plot is reuse of the top panel’s second plot from Extended Data Fig. 1h. b, HaloTag and Halo-CTD fusions bulk wide-field staining using JFX650 in live cell. Scale bar: 1.0 μm. c, Nucleoplasm/Nucleolus ratios for freely diffusing HaloTag and Halo-CTD fusions (n = 100 resamplings; mean value ± s.d.). d, Protein disorder affects hydrodynamic radius (Rh). e, Theoretical relationship between molecule weight and Rh for folded and unfolded peptides (data from Fluidic Analytics). f, g, f, Dfree and estimated Rh of the HaloTag and Halo-CTD fusions in nucleoplasm and nucleolus. Data fitted with Stokes-Einstein equation to calculate g, apparent viscosity (η) of yeast nucleoplasm (NPL) and nucleolus (NOL), respectively. KB: Boltzmann’s constant. T: Temperature in Kelvin; 295.15 K (22 °C) (n = 100 resamplings; mean value ± s.d.). h, f180/0 plot for HaloTag and Halo-CTD fusions (n = 100 resamplings; mean value ± s.d.). Source numerical data are available in source data.

Source data

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