Fig. 1: Glucose deprivation increases lipolysis via ATGL stabilization in the adipose depots and liver.
From: Glucose controls lipolysis through Golgi PtdIns4P-mediated regulation of ATGL
a,b, Quantification of plasma FFA levels (a) and ATGL levels (b) in the liver and adipose depots after 24 h fasting (n = 6 mice per group). c, Plasma FFA levels in the mice 1 h, 6 h and 12 h after 2-DG (2 g kg−1 BW) administration (1 h and 6 h, n = 6 mice for saline and 8 mice for 2-DG group; 12 h, n = 10 mice for saline and 13 mice for 2-DG group). d, Quantification of ATGL levels in the liver and adipose depots collected 1 h, 6 h and 12 h after 2-DG administration (1 h and 6 h, n = 6 mice for saline and 8 mice for 2-DG group; 12 h, n = 12 mice for saline and 15 mice for 2-DG group). e, Representative immunofluorescence of ATGL in the iBAT collected 6 h after 2-DG administration. Scale bar, 20 μm. Representative images from three mice per group with similar results. f, Plasma FFA levels in the wild-type mice after 2-DG treatment for 5.5 h, followed by insulin (0.75 U kg−1 BW) administration for 30 min (n = 8 mice per group). g, Plasma FFA levels in the wild-type mice 6 h after co-administration of 2-DG and insulin (n = 8 mice per group). h,i, Quantification of ATGL and HSLSer660ph levels in the subcutaneous adipose tissue taken before and after EHC from patients with insulin sensitivity or resistance (n = 16 patients with insulin sensitivity in h; n = 8 patients with insulin resistance in i). Data are presented as mean ± s.e.m. and analysed using two-tailed unpaired t-test (a–d (1 h in liver, iBAT, 1 h and 6 h in iWAT, and 1 h and 6 h in gWAT)), two-tailed paired t-test (i), two-tailed Mann–Whitney test (d (6 h and 12 h in liver, 12 h in iWAT and 12 h in gWAT)), two-tailed Wilcoxon test (h) and one-way ANOVA method with Tukey correction (f and g). Source numerical data are available in .
