Extended Data Fig. 5: Glucose deprivation elevates ATGL protein in the Golgi via PtdIns4P regulation.
From: Glucose controls lipolysis through Golgi PtdIns4P-mediated regulation of ATGL
(a) Immunoblot of ectopically expressed ATGL in the HepG2 cells 24 h after treatment with 5 μM PIK93, 10 μM BF738735 and 10 μM UCB9608 (n = 3 independent experiments per group). (b) Quantification of Atgl transcript levels in the iBAs 72 h after Pi4kb knockdown (n = 4 independent experiments per group). (c) Quantification of Atgl transcript levels in the iBAs 72 h after Sacm1l knockdown (n = 4 independent experiments per group). (d) Representative images of SACM1L-EGFP and SACM1LK2A-EGFP in the HEK293-AAV cells. Scale bar, 5 μm. Two times repeated independently with similar results. (e) Immunoblot of ATGL in the HEK293T cells 6 h after treatment with glucose free medium under the background of SACM1LK2A-EGFP overexpression (n = 6 independent experiments per group). (f) Immunoblot of ATGL in the HEK293T cells 6 h after glucose deprivation under the background of UCB9608 treatment (n = 6 independent experiments per group). Results are shown as mean ± s.e.m. and analyzed using two-tailed unpaired t-test (e and f) and one-way ANOVA method with Dunnett correction for multiple comparisons between control and other groups (a to c). Source numerical data and unprocessed blots are available in source data.
