Extended Data Fig. 2: Glucose deprivation elevates systemic lipolysis via upregulating ATGL in the adipose depots independently of transcriptional changes.
From: Glucose controls lipolysis through Golgi PtdIns4P-mediated regulation of ATGL
(a) Immunoblots of ATGL, HSLSer660ph, HSL, AKT1, AKT1Ser473ph and HSP90 in the gWAT from wild-type mice after 2-DG (2 g/kg BW) treatment for 5.5 h, followed by insulin (0.75 U/kg BW) administration for 30 minutes (n = 6 mice per group). (b) Quantification of Atgl transcript levels in the liver and adipose depots from fasted mice via qPCR (n = 6 mice per group). (c) Quantification of Atgl transcript levels in the liver and adipose depots collected 1 h after 2-DG (2 g/kg BW) administration (n = 6 mice for saline and 8 mice for 2-DG group). (d) Quantification of Atgl transcript levels in the liver and adipose depots collected 6 h after 2-DG (2 g/kg BW) administration (n = 6 mice for saline and 8 mice for 2-DG group). (e–f) Immunoblot of ATGL in the liver and determination of plasma FFA levels in liver-specific Atgl knockout mice (AtglLKO) 6 h after 2-DG (2 g/kg BW) administration (WT: n = 8 mice for saline and 7 mice for 2-DG group; AtglLKO mice: n = 7 mice per group). (g–h) Immunoblot of ATGL in the iBAT and determination of plasma FFA levels in adipose tissue-specific Atgl knockout mice (AtglAKO) 6 h after 2-DG (2 g/kg BW) administration (WT: n = 4 mice for saline and 5 mice for 2-DG group; AtglAKO: n = 6 mice for saline and 5 mice for 2-DG group). Results are shown as mean ± s.e.m. and analyzed using two-tailed unpaired t-test (b, c (liver, iBAT and iWAT), d, f (WT) and h), two-tailed Mann-Whitney test (c (gWAT) and f (AtglLKO)) and one-way ANOVA method with Tukey correction (a). Source numerical data and unprocessed blots are available in source data.
