Extended Data Fig. 6: Stalled transcription bodies go into productive elongation in mir430-/- + inj MiR430 embryos.
From: Transcription bodies regulate gene expression by sequestering CDK9
Visualization of initiating (Ser5P) and elongating (Ser2P) RNAPII with Fabs in WT (a, c) and mir430-/- + inj MiR430 (b, d) embryos at 512-cell stage (a, b) and 1024-cell stage (c, d). Shown are representative images of individual nuclei, extracted from a spinning disk confocal microscopy timelapse. The micrographs shown in the main figure are highlighted in green. N = 3 biologically independent experiments. Information on quantification can be found in the legend of Fig. 5b.
