Extended Data Fig. 10: MiR430 injection only modestly affects the number of ectopic transcription bodies.
From: Transcription bodies regulate gene expression by sequestering CDK9
Quantification of ectopic transcription bodies in WT + inj MiR430. Data for WT, WT + inj cdk9, and mir430-/- + inj MiR430 are the same as in Fig. 6g. N = 3 biologically independent experiments, n = 101, 184, 290 and 378 at 128c, 256c, 512c and 1024c, respectively in WT. n = 43, 82, 127 and 179 at 128c, 256c, 512c and 1024c, respectively in WT + inj cdk9. n = 45, 65, 97 and 152 at 128c, 256c, 512c and 1024c, respectively in mir430-/- + inj MiR430. n = 14, 33, 75, 92 at 128c, 256c, 512c and 1024c, respectively in WT + inj MiR430. Boxplots show median, quartiles, minimum and maximum, and 1.5x interquartile range. Individual points represent outliers. At each stage, Kruskal-Wallis tests were performed (128c: χ2 = 15.685, df = 3, P-value = 0.001315; 256c: χ2 = 107.17, df = 3, P-value < 2.2 × 10−16; 512c: χ2 = 219.57, df = 3, P-value < 2.2 × 10−16; 1024c: χ2 = 413.81, df = 3, P-value < 2.2 × 10−16). When this test was statistically significant (P-value < 0.05), pairwise comparisons with Bonferroni correction were performed using a pairwise Wilcoxon rank-sum test. A comparison was considered significant when adjusted P-value < 0.05, and adjusted P-values were reported using WT as reference. See Methods for a detailed description of quantification. Source numerical data are available in source data.
