Fig. 2: CSB acts in parallel to known DPC repair pathways in promoting DPC tolerance. | Nature Cell Biology

Fig. 2: CSB acts in parallel to known DPC repair pathways in promoting DPC tolerance.

From: Transcription-coupled repair of DNA–protein cross-links depends on CSA and CSB

Fig. 2

a, Alamar blue cell viability assays with the indicated CSB- and SPRTN-deficient RPE1 cell line clones treated with formaldehyde. b,c, Alamar blue assays with the indicated cell lines treated with formaldehyde after siCSB or a non-targeting siRNA control (siCtrl) in WT versus SPRTN-ΔC cells (b) or siSPRTN in WT versus CSB−/− RPE1 cells (c). d,e, Alamar blue assays in the WT versus CSB−/− RPE1 cells upon RNF4 depletion, treated with formaldehyde (d) or 5-aza-dC (e). In a–e, symbols and error bars denote mean ± s.d., n = 3 replicates. f,g, Clonogenic survival assays in WT or CSB−/− HAP1 cells upon siRNA-mediated depletion of RNF4, treated with formaldehyde (f) or 5-aza-dC (g). Symbols and error bars denote mean ± s.e.m., n = 3 replicates. Source numerical data are available in Source data.

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