Fig. 5: Upstream TC-NER factors support transcription recovery after DPC induction.
From: Transcription-coupled repair of DNA–protein cross-links depends on CSA and CSB
a,b, RRS assays in WT, CSB−/− or CSA−/− RPE1 cells treated with formaldehyde (a) or UVC (b) and released for the indicated times. c, The same as for a but with WT, CSB−/− and ELOF1−/− RPE1 cells. d, The same as for a but with WT, CSB−/− and RPB1K1268R HeLa cells. e,f, The same as for a and b but with WT, CSB−/− and UVSSA−/− RPE1 cells. g,h, The same as for a and b but with XPC−/−, XPC−/−/CSB−/− and XPC−/−/XPA−/− RPE1 cells. i,j, The same as for a and b but with WT, CSB−/−, ERCC1−/− and XPG−/− RPE1 cells. Mean intensities of replicates (n = 3 for a, b, e, g and i; n = 5 for c; and n = 4 for d) are displayed as black dots with a mean of those averages shown, error bars ± s.e.m. For f, h and j, n = 2 and mean intensities of individual replicates are shown as black dots and the mean of those values is shown as a black line. Note that for c, data from WT and CSB−/− cells are shared with some replicates from other images shown in this figure. Source numerical data are available in Source data.
