Extended Data Fig. 7: CD32 is expressed in WNT-independent hPSC-derived HECs.
From: CD32 captures committed haemogenic endothelial cells during human embryonic development
a) Representative flow cytometric analysis showing the gating strategy to isolate CD32+ (orange) and CD32neg (blue) cells from day 8 of WNTi hPSC-derived. From the left, first panel: gated on SSC/FSC/Live. Second panel: gated on SSC/FSC/Live/CD34+CD43neg. Third and fourth panels: gated on SSC/FSC/Live/CD34+CD43negCD184negCD73neg. Fourth panel: unstained control. n = 7, independent; b) Bar plot showing the frequency of CD32+/negDLL4+/neg cells within day 8 of WNTi hPSC-derived CD34+CD43negCD184negCD73neg cells. Gated on SSC/FSC/Live/CD34+CD43negCD184negCD73neg. n = 7, independent, mean ± SEM; c) Quantification of erythro-myeloid CFC potential of CD32+/neg populations isolated at day 8 of WNTi hPSC-derived hematopoietic cultures. Mean ± SEM. One-tail paired Student’s t-test, nonparametric, for all biological replicates (n = 6, H1 hESCs), considering the total number of colonies, *p = 0.0111; d) Representative flow cytometric analysis showing CD45+CD56+ NK-cells derived from CD32+ cells isolated at day 8 of WNTi hPSC-derived hematopoietic culture. Gated on SSC/FSC/Live. H1 hESCs. n = 3, independent.
