Fig. 2: CD32 is expressed in AGM and YS HECs during human embryonic development.
From: CD32 captures committed haemogenic endothelial cells during human embryonic development
a, A heatmap of top ten differentially expressed surface genes within human embryonic ACE+ (light blue) and ACEneg (beige) cells derived from four CS12–CS13 human embryos (E1, E2, E3 and E4). rlog gene expression values are shown in rows. Colouring indicates differential expression by upregulation (red) or downregulation (blue). b, CD34 (top) and CD32 (bottom) expression by immunohistochemistry of consecutive sections of the AGM of a 29 dpf (CS13) human embryo (n = 5 independent). Inset shows high magnification of haematopoietic clusters (black arrowhead) and surrounding endothelial cells (white arrowhead) immunostained by CD32. Ao, aorta. Scale bars, 50 μm and 25 μm (inset). c, Transverse consecutive sections of the AGM region of a 29 dpf (CS13) human embryo, immunostained with CD34 (green), CD32 (red) and merge (top, from left to right) and RUNX1 (green), ACE (red) and merge (bottom, from left to right). Scale bars, 50 μm, n = 3 independent. d, CD34 (top) and CD32 (bottom) expression by immunohistochemistry of consecutive sections of the YS of a 26 dpf (CS12) human embryo (n = 5 independent). Scale bars, 25 μm. e,f, Flow cytometric analysis (e) and quantification of erythro-myeloid CFC potential (f) of CD32+ (orange) and CD32neg (blue) cell populations isolated from the AGM and YS of CS13 human embryos (E5 and E6). n = 2, independent. PE, phycoerythrin; PE-Cy7, phycoerythrin-cyanine7; BFU-E, burst-forming unit erythroid; CFU-GM, colony-forming unit granulocyte macrophage; CFU-M, colony-forming unit macrophage.
