Extended Data Fig. 2: Identification of CD32 in the AGM and the YS of human embryos.
From: CD32 captures committed haemogenic endothelial cells during human embryonic development
a) Volcano plot showing the differentially expressed cell surface genes in ACE+ vs ACEneg cells isolated from the AGM of four CS12-CS13 human embryos (Wald test; FDR < 0.05). The top 10 differentially expressed cell surface genes are highlighted, upregulated in red, downregulated in blue, nonsignificant in grey; b) Experimental layout: CD34+CD43negCD45negCD32+/neg (referred to as CD32+ and CD32neg) cells were FAC-sorted from the AGM and YS of two CS13 human embryos. Isolated cells were tested for their hematopoietic potential via CFC generation assay; c) Representative flow cytometric analysis showing the gating strategy to isolate CD32+ (orange) and CD32neg (blue) cells from the AGM of CS13 human embryo. From the left, first panel: gated on SSC/FSC/Live. Second panel: gated on SSC/FSC/Live/CD34+CD43neg. Third panel: gated on SSC/FSC/Live/CD34+CD43negCD45neg. Fourth panel: unstained control, gated on SSC/FSC/Live. n = 2, independent; d) Representative flow cytometric analysis showing the gating strategy to isolate CD32+ (orange) and CD32neg (blue) cells from the YS of CS13 human embryo. n = 2, independent. From the left, first panel: gated on SSC/FSC/Live. Second panel: gated on SSC/FSC/Live/CD34+CD43neg. Third panel: gated on SSC/FSC/Live/CD34+CD43negCD45neg. Fourth panel: unstained control, gated on SSC/FSC/Live.
