Extended Data Fig. 3: RSL3-/erastin-resistant cells prefer SFA into membrane PL.
From: LPCAT1-mediated membrane phospholipid remodelling promotes ferroptosis evasion and tumour growth
a, Heatmaps showing the metabolite content as detected by metabolomics. b–d, Representative images (b, c) and quantification (d) of membrane azide-fluor 488 fluorescence intensity in the indicated cells treated with PA-alkyne (5 μM, 10 h), or AA-alkyne (5 μM, 10 h), or ALA-alkyne (5 μM, 10 h), or AA-alkyne (5 μM, 10 h) normalized against integrin β1 fluorescence intensity. Scale bar, 10 μm or 2 μm. e-g, Quantification of RSL3- or erastin-induced membrane Liperfluo fluorescence intensity in the indicated cells treated with different FAs normalized against Dil intensity. h, Viability analysis of the indicated cells treated with different FFAs plus RSL3 or erastin for 24h. Data are presented as mean ± SD, n = 3 biologically independent samples in a, h, n = 6 biologically independent samples in b-g. Statistical analysis was performed using an unpaired two-tailed Student’s t-test in h, and one-way ANOVA followed by Dunnett’s test in d-h. NS, not significant.
